논문검색
Author Eun Kyoung Yim, Jun Sang Bae, Seung Bak Lee, Keun Ho Lee, Chan Joo Kim, Sung Eun Namkoong, Soo Jong Um, Jong Sup Park
Place of duty 1Department of Medical Bioscience, Graduate School of Catholic University, 2Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Catholic University Medical College, 3Department of Bioscience and Biotechnology/Institute of Bioscience, Sejong University, Republic of Korea.
Title Proteome Analysis of Differential Protein Expression in Cervical Cancer Cells after Paclitaxel Treatment
Publicationinfo Cancer Research and Treatment 2004 Dec; 036(06): 395-399.
Key_word Paclitaxel,HPV,Cervical carcinoma,Proteomics,Apoptosis
Full-Text
Abstract Purpose: It is well known that infection with HPV (human papillomavirus) is the main cause of cervical cancer and certain types of HPV are recognized as carcinogens. At present, there is little information regarding the antineoplastic mechanism of paclitaxel against cervical carcinoma cells. We thus tried to analyze differential protein expression and antineoplastic mechanism-related proteins after paclitaxel treatment on cervical cancer cells by using a proteomic analysis and to investigate the mechanism of action. Materials and Methods: Using proteomics analysis including 2-DE and MALDI-TOF-MS, we detected the antineoplastic mechanism-related proteins. Then, we performed western blot analysis for apoptosis- and transformation- related proteins to confirm expression patterns derived from proteome analysis after paclitaxel treatment. Results: We identified several cellular proteins that are responsive to paclitaxel treatment in HeLa cells using proteomics methods. Paclitaxel treatment elevated main-ly apoptosis, immune response and cell cycle check point- related proteins. On the other hand, paclitaxel treatment diminished growth factor/oncogene-related proteins and transcription regulation-related proteins. Also, in the HPV-associated cervical carcinoma cells, paclitaxel demonstrated anti-proliferative activity through the membrane death receptor-mediated apoptotic pathway and the mitochondrial-mediated pathway. Conclusion: Identification and characterization of functionally modulated proteins involved in anti-cancer regulatory events should lead to a better understanding of the long-term actions of paclitaxel at the molecular level and will contribute to the future development of novel therapeutic drug treatments based upon current therapies. (Cancer Research and Treatment 2004;36:395-399)